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KMID : 0379119910190040276
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Korean Journal of Mycology
1991 Volume.19 No. 4 p.276 ~ p.281
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Comparative studies on xylose utilization and fermentation by Pachysolen tannophilus fusants and auxotrophs
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Abstract
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Auxotrophs induced by UV irradiation were lysine, arginine, methionine, and cysteine requirement. The optimal protoplast-producing conditions of Pachysolen tannophilus were obtained in 1 hr incubation treated zymolyase 0.5 ㎎/㎖ at 37-42℃(pH 6.5-8.5 with 0.6 M KCl as osmotic stabilizer. Cell volumn of fusants were counted in about 4 times of parents and their morphological characteristics were similar to parents which were global or egg-shaped, though in fusant, pseudohypha were observed frequently. Ethanol production of fusants were similar to parents, but arginine and methionine auxotrophs were showed increased ethanol production respectively 1.6 times and 1.25 times more than parents.
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